Our Technology – Kcat Enzymatic

SCALE-UP FRAMEWORK FOR INDUSTRIAL
ENZYME/PROTEIN DEVELOPMENT®

In-Silico Guided CRISPR-Cas9 Driven Enzyme Engineering Technology (Kit); Easy And Fast Way For Engineering Enzymes

Salient features of Kcat Enzymatic CRISPR-Cas9 Enzyme EngineeringTechnology (Kit): The source gene (enzyme) of interest will be provided by Kcat Enzymatic. The kit contains

Proprietary CRISPR-Cas9 enzymes with the customized vectors, sgRNAs, and PAM that can do both specific and random mutations based on which the hotspots and regions to be mutated will be given by Kcat Enzymatic

The customers can also suggest the source gene, hotspots and regions

An AI algorithm is included along with the kit to predict hotspots and regions for generating the next set of focused libraries

Affordable and can be executed in a reasonably equipped laboratory; bypassing gene synthesis

Mechanics Of Kcat Enzymatic’s Enzyme Engineering Framework

Still, most enzyme engineering project relies on directed evolution although it is an expensive affair. Our aim is to disrupt this and make engineering very much possible with fewer experiments. On average, it takes a minimum of 5000 enzyme variants to be tested in the lab to make an enzyme that works on the industrial scale and that “makes a business”. “This is the biggest bottleneck”. What if we could do it with fewer experiments? In one of the projects, we achieved this by testing just 70 variants. We have developed algorithms and these show >75% predictability, notably high scalability up to quadruplet mutants. Beyond 4 mutations we have achieved 60 % predictability. Here we are listing a glimpse of the technologies that we have developed specifically for engineering enzymes.

7D GRID ENZYME ENGINEERING TECHNOLOGY ®

Salient features of Kcat Enzymatic CRISPR-Cas9 Enzyme EngineeringTechnology (Kit): The source gene (enzyme) of interest will be provided by Kcat Enzymatic. The kit contains

Proprietary CRISPR-Cas9 enzymes with the customized vectors, sgRNAs, and PAM that can do both specific and random mutations based on which the hotspots and regions to be mutated will be given by Kcat Enzymatic

The customers can also suggest the source gene, hotspots and regions

An AI algorithm is included along with the kit to predict hotspots and regions for generating the next set of focused libraries

Affordable and can be executed in a reasonably equipped laboratory; bypassing gene synthesis