The Greenaldolase® is unique in catalyzing an aldol reaction between two aldehydes, and its broad substrate specificity confers considerable utility as a biocatalyst, offering an environmentally benign alternative to chiral transition metal catalysis of the asymmetric aldol reaction.

Several variants have undergone pilot-scale testing, exhibiting greater than 90% activity with 99% e.e. at substrate concentrations of 30-50 g/L, temperatures exceeding 55 °C, pH values exceeding 8.0, and acetaldehyde tolerance of 50-60 g/L