Mechanics Of Kcat Enzymatic’s Enzyme Engineering Framework

Still, most enzyme engineering project relies on directed evolution although it is an expensive affair. Our aim is to disrupt this and make engineering very much possible with fewer experiments. On average it takes a minimum of 5000 enzyme variants to be tested in the lab to make an enzyme that works on the industrial scale and that “makes a business”. “This is the biggest bottleneck”. What if we could do it with fewer experiments? In one of the projects, we achieved this by testing just 70 variants. We have developed algorithms and these show >75% predictability, notably high scalability up to quadruplet mutants. Beyond 4 mutations we have achieved 60 % predictability. Here we are listing a glimpse of the technologies that we have developed specifically for engineering enzymes.