Identification of a Reaction Intermediate and Mechanism of Action of Intermediary Enzymes in Plumbagin Biosynthetic Pathway

Scaling up is a problem that a lot of biotechnologists find difficult, in terms of manufacturing techniques and products; that’s where we provide biocatalytic solutions at Kcat! The above schema is an intermediary reaction in the Plumbagin Biosynthetic Pathway. We at Kcat have identified two critical steps and respective enzymes in the synthesis of Plumbagin, the yellow dye with multitudinous medicinal benefits. The missing intermediates have been predicted by our scientists using QM studies and 3D based database screening to help identify the enzymes. The highlight is, all this is achieved especially when the genome of the organism is yet to be sequenced. https://doi.org/10.3390/catal10030280