In-Depth Sequence–Function Characterization Reveals Multiple Pathways To Enhance Enzymatic Activity

May 13, 2022by Kcat-Admin

Phenyl Ammonia Lyase (PAL) has garnered significant attention as the active ingredient in Pegvaliase, the only FDA-approved drug for treating classical phenylketonuria (PKU).


Deep Mutational scanning (DMS) to derive functional hotspots
Hybrid QM/MM techniques combined with meta-dynamics on all 4 active sites of the PAL tetramer simultaneously, to enhance the sampling of coordinates relevant to the reaction

Read more: