The invention describes the methods of directing CRISPR complex formation in bacterial cells and utilizing the CRISPR-Cas system to edit a gene of interest in a plasmid. An AI incorporated CRISPR tool to engineer enzymes for better activity and allow cells to undergo specific and random mutation for a variety of research or translational applications, and modified vector systems that encode single or multiplex gene targets and cas9/deaminase cas9 proteins are also disclosed.
Patent No: 544989
Date of grant: 15-07-2024