The functional repurposing of structural proteins offers exciting opportunities for sustainable and efficient biocatalysis. This study presents the engineering of two structural proteins, F-actin, to exhibit nitrilase activity, enabling the conversion of 1-(cyanomethyl)cyclohexane-1-carbonitrile into 2-(1-cyanocyclohexyl)acetic acid, a key intermediate in the production of gabapentin. By introducing catalytic residues adapted from nitrilase enzymes, these engineered proteins...
The present invention describes identifying if a given Transaminase is (R)-Selective or (S)-Selective. Based on the binding of the substrate in the active site of a given transaminase, it is primarily based on torsion, the angle between specific atoms of the cofactor PLP and the catalytic Lysine, and the ten interactions of the substrate with...
The invention describes the methods of directing CRISPR complex formation in bacterial cells and utilizing the CRISPR-Cas system to edit a gene of interest in a plasmid. An AI incorporated CRISPR tool to engineer enzymes for better activity and allow cells to undergo specific and random mutation for a variety of research or translational applications,...
